Figure 4.

Effect of chronic low‐dose aspirin treatment on colon tumor formation and inflammation in the AOM model. (A) Schematic representation of the AOM and aspirin treatment protocol. (B) Tumor number, (C) tumor size, and (D) plasma TXB₂ concentration of mice (n = 9/group) following 17 wks of aspirin administration. (E and F) Colon length (E) and disease activity index (F) of control and aspirin‐treated mice (n = 9/group). (G) Representative pictures of immunohistochemical staining for the macrophage marker F4/80 of colon sections (magnification ×100) and quantification of the number of positive cells from control and aspirin‐treated mice (n = 3‐4/group). (H and I) IL‐1β (H) and IL‐6 (I) concentration in supernatants from colon explant cultures of control and aspirin‐treated mice (n = 5/group). **P < .01 by unpaired Student's t test