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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Curr Opin Chem Biol. 2019 Nov 1;54:1–9. doi: 10.1016/j.cbpa.2019.09.004

Figure 2. GFP phase separation-based activity reporter of kinases.

Figure 2.

(a) Top, DNA construct of SPARK. Bottom, cartoon showing two parts of the SPARK. HOTag (homo-oligomeric tag) is de novo designed coiled coils (~30 amino acids). (b) Schematic showing multivalent interaction-driven phase separation of SPARK upon kinase phosphorylation. Right panel, fluorescence images of a HEK293 cell expressing PKA-SPARK upon protein kinase A (PKA) activation. Adapted with permission from reference30. Copyright 2019 Elsevier Inc.