Extended Data Fig. 2. Learning impairment lasts over a period of 5 days in PAE mice.

(a) The accelerated rotarod test was performed with the same daily schedule as shown in Fig. 1c for 5 days. PAE mice show significantly shorter latency to fall compared to control. F(1,19) = 28.3, P = 0.0001 by two-way repeated measures ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Tukey’s test (control: n = 11 animals, PAE: n = 10 animals). Graph shows mean ± SEM. (b) Learning index over the 5-day period is lower in PAE mice. *P = 0.015 by two-tailed Student’s t-test (control: n = 11 animals, PAE: n = 10 animals). (c) The accelerated rotarod test was performed for 5 days with 3-month-old animals. No significant differences were observed in body weight between control and PAE mice; P = 0.243 by two-tailed Student’s t-test (control: n = 12 animals, PAE: n = 14 animals). (d) PAE mice show significantly shorter latency to fall compared to control mice. F(1,24) = 11.57, P = 0.003 by two-way repeated measures ANOVA. *P < 0.05 by Tukey’s test (control: n = 12 animals, PAE: n = 14 animals). Graph shows mean ± SEM. (e) Learning index over the 5-days period is also lower in PAE mice. *P = 0.026 by two-tailed Student’s t-test (control: n = 12 animals, PAE: n = 14 animals). In box plots (b, c, e), the line within the box indicates the median, and the upper and lower edges of the box represent the 25th and 75th percentiles, respectively. The upper and lower whisker boundaries indicate the 10th and 90th percentiles, respectively, and dots indicate outliers.