Table 1:

Primers information

Gene Name	Accession Number	Primer Sequence	Amplicon Length	Annealing Temperature
Interleukin 1β (Il1b)	NM_031512.2	F: 5’ – TGTGATGAAAGACGGCACAC – 3’ R: 5’ – TGTGCTCTGCTTGAGAGGTGCT – 3’	176	60.2 ºC
Tumor necrosis factor (Tnf)	NM_012675.3	F: 5’ – CCGACTCTGACCCCCATTAC – 3’ R: 5’ – CCCAGAGCCACAATTCCCTT – 3’	98	60.2 ºC
Interleukin 6 (Il6)	NM_012589.2	F: 5’ – ATGAGGTCTACTCGGCAAAC – 3’ R: 5’ – TCTGACCACAGTGAGGAATG – 3’	75	60.2 ºC
Interferon gamma (Ifng)	NM_138880.2	F: 5’ – AGTCTGAAGAACTATTTTAACTCAAGTAGCAT – 3’ R: 5’ – CTGGCTCTCAAGTATTTTCGTGTTAC – 3’	117	60.2 ºC
Interleukin 10 (Il10)	NM_012854.2	F: 5’ – TGCGACGCTGTCATCGATTT – 3’ R: 5’ – AGACACCTTTGTCTTGGAGCTT – 3’	96	61.8 ºC
Interleukin 4 (Il4)	NM_201270.1	F: 5’ – AACAAGGAACACCACGGAGAA – 3’ R: 5’ – TCTTCAAGCACGGAGGTACA – 3’	95	62.2 ºC
Glyceraldehyde-3-Phosphate Dehydrogenase (Gapdh)	NM_017008.4	F: 5’ – TGACAAAGTGGACATTGTTGC – 3’ R: 5’ – CTTGCCGTGGGTAGAGTCAT – 3’	91	60.2 ºC, 61.8 ºC, 62.2 ºC