Figure 2.

Effects of FoxO1 by evaluating bodyweight, DAI score and pathological condition of mice with chronic colitis. A, The intestinal histopathological scores in mice showed that the pathological scores were 0 in FoxO1‐WT‐Con and FoxO1‐Tg‐Con groups. However, after DSS intervention, the pathological scores were significantly higher in FoxO1‐Tg‐DSS group than those of FoxO1‐WT‐DSS group. Comparison between groups, ^* P<.05. B, The change in intestinal tissue length in mice: The length of intestinal tissue was significantly lower in FoxO1‐Tg‐DSS group than that in FoxO1‐WT‐DSS group. Comparison between groups, ^* P<.05. C, DAI scores in mice showed no significant changes in FoxO1‐WT‐Con and FoxO1‐Tg‐Con mice during the experiment, whereas DAI scores were significantly changed in FoxO1‐Tg‐DSS and FoxO1‐WT‐DSS groups. Comparison with FoxO1‐WT‐DSS group at the same time‐point, ^* P<.05. D, Bodyweight changes of mice: The bodyweight was gradually increased in FoxO1‐WT‐Con and FoxO1‐Tg‐Con mice under normal feeding, without significant difference. However, the bodyweight was decreased in FoxO1‐Tg‐DSS and FoxO1‐WT‐DSS groups. Comparison with the FoxO1‐WT‐DSS group at the same time‐point, ^* P<.05. E, HE staining showed that the intestinal mucosa epithelium was intact, and the intestinal gland composed of lamina propria and mucosa muscle layer was arranged regularly in FoxO1‐WT‐Con group under light microscope. However, the colonic mucosa was defective, with decreased goblet cells and destructive or even disappeared gland, and large number of lymphocytes were infiltrated in the submucosa and even the muscle layer in FoxO1‐WT‐DSS group, which was more severe in FoxO1‐Tg‐DSS group than FoxO1‐WT‐DSS group. F,G, The expression of FoxO1 in mouse intestinal tissue: The expression of FoxO1 was increased in FoxO1‐WT‐DSS and FoxO1‐Tg‐DSS groups after DSS intervention, and the expression of FoxO1 was significantly higher in FoxO1‐Tg‐DSS group than that in FoxO1‐WT‐DSS group. Comparison between groups, ^* P < .05