Figure 6.

Cellular expression of endothelial cell and renal tubular injury biomarkers by day 60 after CKD induction. A to E, Illustrating the immunofluorescent (IF) microscopic finding (400x) for identification of CD31⁺ cells (green colour). F, Score of CD31 cell expression, * vs other groups with different symbols (†, ‡, §), P < .0001. G to K, Illustrating the IF microscopic finding (400×) for identification of positively stained kidney injury molecule (KIM) cells (green colour). L, Score of KIM cell expression, * vs other groups with different symbols (†, ‡, §), P < .0001. Red colour in B, D, E, H, J and K indicated some residual iPS‐MSC^SPIONs kidney parenchyma. Scale bars in right lower corner represent 50µm. All statistical analyses were performed by one‐way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6 for each group). Symbols (*, †, ‡, §) indicate significance (at 0.05 level). G1 = sham‐operated control (SC); G2 = SC + iPS‐MSC^SPIONs (1.0 × 10⁶ cells); G3 = CKD; G4 = CKD + iPS‐MSC^SPIONs (0.5 × 10⁶ cells); G5 = CKD + iPS‐MSC^SPIONs (1.0 × 10⁶ cells). CKD, chronic kidney disease; iPS‐MSC, induced pluripotent stem cells‐derived mesenchymal stem cells; iPS‐MSC^SPIONs, the iPS‐MSC was labelled by paramagnetic contrast agent