Figure 10.

Kidney injury score and fibrotic area in kidney parenchyma by day 60 after CKD induction. A to E, Light microscopic findings of haematoxylin and eosin. stain (400×) demonstrating significantly higher degree of loss of brush border in renal tubules (yellow arrows), tubular necrosis (green arrows), tubular dilatation (red asterisk) protein cast formation (black asterisk) and dilatation of Bowman's capsule (blue arrows) in IR group than in other groups. F, * vs other groups with different symbols (†, ‡, §), P < .0001. G to K, Immunofluorescent stain (200×) for identification of fibrosis in kidney parenchyma (blue colour). L, Analytical results of fibrotic area, * vs other groups with different symbols (†, ‡, §), P < .0001. Scale bars in right lower corner represent 50µm. All statistical analyses were performed by one‐way ANOVA, followed by Bonferroni multiple comparison post hoc test (at least n = 6 for each group). Symbols (*, †, ‡, §) indicate significance (at .05 level). G1 = sham‐operated control (SC); G2 = SC + iPS‐MSC^SPIONs (1.0 × 10⁶ cells); G3 = CKD; G4 = CKD + iPS‐MSC^SPIONs (0.5 × 10⁶ cells); G5 = CKD + iPS‐MSC^SPIONs (1.0 × 10⁶ cells). CKD, chronic kidney disease; iPS‐MSC, induced pluripotent stem cells‐derived mesenchymal stem cells; iPS‐MSC^SPIONs, the iPS‐MSC was labelled by paramagnetic contrast agent