Figure 2.

PANK1 was up‐regulated in liver tissue of mice on high‐fat diet and in cultured hepatocytes treated with palmitate acid. A, The mice were fed on HFD, and the protein levels of PANK1, PANK2 and PANK3 in the liver tissue were measured by Western blotting (n = 6). B, The AML12 cells were treated with 0, 50, 100, 200, 400, 600 and 800 μmol/L palmitate acid (PA) for 24 hours, and the protein level of PANK1 was measured by Western blotting (n = 3 independent experiments). PANK1 vector or blank vector was transfected into AML12 cells with lipofectamine. The mRNA level of G6Pase (C) and PEPCK (D) in AML12 cells were measured by real‐time RT‐PCR. The activity of G6Pase (E) and PEPCK (F) and glucose production (G) was measured in AML12 cells. The protein levels of FASN, SCD1 and Cpt1α were measured by Western blotting (H). I, Before harvest, cells were stimulated with 100 nmol/L insulin for 20 min and protein expressions of p‐Akt (S473) and Akt were measured by Western blotting. All values are presented as mean ± SEM. Data in A and B, *P < .05, **P < .01, ***P < .001 (vs CON). Data in the rest, *P < .05, **P < .01 (vs Blank vector), n = 3 independent experiments