Figure 3.

Knockdown PANK1 ameliorated metabolic reprogramming but failed to improve insulin resistance induced by palmitate acid. The AML12 cells were treated with scrambled small interfering RNA (si Scr) or PANK1 small interfering RNA (si PANK1). After 24 h, the cells were exposed to palmitate acid (600 nmol/L, PA) for 24 h. A and B, The mRNA level of G6Pase and PEPCK in AML12 cells was measured by real‐time RT‐PCR. C and D, The activity of G6Pase and PEPCK in AML12 cells was measured. E, Glucose production measured in AML12 cells. F, The protein levels of FASN, SCD1 and Cpt1α in AML12 cells were measured by Western blotting. G, Before harvest, cells were stimulated with 100 nmol/L insulin for 20 min and protein expressions of p‐Akt (S473) and Akt were measured by Western blotting. All values are presented as mean ± SEM. **P < .01, ***P < .001 (vs si Scr); ^# P < .05, ^## P < .01, ^### P < .001 (vs PA + si Scr), n = 3 independent experiments