Figure 3.

Effect of Mel on the expression of YAP in Dox‐treated H9c2 cells. Representative Western blot images (A) and quantification of Yes‐associated protein 1 (YAP) expression (B) in control, doxorubicin (Dox), melatonin (Mel) and Mel with Dox co‐treated H9c2 cells. (C) Representative fluorescent images showing YAP expression in the 4 groups of the H9c2 cells. Real‐time qPCR quantification of YAP target genes: Connective tissue growth factor (CTGF, D), baculoviral IAP repeat containing 5 (birc5, E) and parkin RBR E3 ubiquitin protein ligase (Park2, F) in the 4 groups of the cells. β‐actin was used as a house‐keeping protein. n = 3 independent experiments/group. *P < .05 compared with the control group, **P < .01 compared with the control group, ^# P < .05 compared with the Dox‐treated group, ^## P < .05 compared with the Dox‐treated group