Figure 5.

Knockdown of YAP reversed the protective effect of Mel on Dox‐ induced apoptosis. Representative images of TUNEL staining (A) and quantification of apoptosis (B) in the control, doxorubicin (Dox), melatonin (Mel), Mel+Dox, Mel+Dox+siNC (control siRNA) and Mel+Dox+siYAP treated H9c2 cells. Representative Western blot images (C) and quantification of Yes‐associated protein 1 (YAP, D), Bax (E) and Bcl‐2 (F) are shown. β‐actin was used as a house‐keeping protein. n = 3 independent experiments/group, *P < .05 compared with the control group, **P < .01 compared with the control group, ^# P < .05 compared with the Dox‐treated group, ^## P < .05 compared with the Dox‐treated group