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Effects of M2 macrophages on cell viability and inflammation in PTECs under H/R. A, Real‐time PCR analysis of Il1b, Il6, Nlrp3 and Il10 mRNA level. B, Western blot analysis of NLRP3 and IL‐1β protein level. (C) Cell viability was determined using the CCK‐8 assay. D, Cell proliferation rates detected by flow cytometry. E and F, The apoptotic ratios of PTECs determined by flow cytometry. *P < .05, **P < .01 vs Control. #P < .05, ##P < .01 vs H/R (n = 5)
