FIGURE 4.

Classification performance using definitively identified metabolites. These receiver operating characteristic (ROC) plots show the classification model performance using the seven MS/MS definitively identified metabolites for the cognitively unaffected and stable (CS) versus Down syndrome‐Alzheimer's disease (DS‐AD) comparison (A) and the two definitively identified metabolites for the DS‐AD versus Down syndrome‐mild cognitive impairment (DS‐MCI) comparison (B). The classification performance from these reduced set of metabolites is not significantly different from the larger sets used in Figure 3. The consistency of the ROC AUC across the resampling schemes (10‐fold CV and 100‐fold Monte Carlo CV) and classification models (logistic regression and support vector machine) shows the overall stability of the models and argues against overfitting