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. 2020 Apr 1;13:2725–2738. doi: 10.2147/OTT.S236359

Figure 7.

Figure 7

DSCAM-AS1 as ceRNA regulated FSTL3 by targeting miR-122-5p. (A) The binding site between DSCAM-AS1 and miR-122-5p. (B) The expression of miR-122-5p and DSCAM-AS1 was detected by qRT-PCR to analyze the correlation of their expressions. (C) The expression of FSTL3 and DSCAM-AS1 was detected by RT-qPCR to analyze the correlation of their expressions. (D) The coexistence of DSCAM-AS1 and miR-122-5p in NSCLC cells was identified by RIP assay. (E) Luciferase reporter gene assay confirmed the binding relationship between DSCAM-AS1 and miR-122-5p. (F) The expression of DSCAM-AS1 in normal bronchial cells 16HBE and 5 NSCLC cell lines was detected by qRT-PCR. *P<0.05, **P<0.01, ***P<0.001, n.s: P>0.05.