Table 2.
Primary and Secondary Antibodies Used for Immunohistochemical Analysis
| Primary Antibody | Dilution and Source | Secondary Antibody and Source | Tissue Fixation | Target | Indicates |
|---|---|---|---|---|---|
| Monoclonal mouse anti rat RECA-1, IgG1 isotype | 1:200; Serotec, UK | Biotinylated rabbit anti-mouse IgG1; Zymed Laboratories, San Francisco, CA, USA | MC | A cell surface antigen expressed by rat endothelial cells | Small and large blood vessels |
| Monoclonal rat CD34 [clone MEC 14.7] IgG2a isotype | 1:50; Abcam | Biotinylated anti-rat IgG2a: Vector Laboratories, Burlingame, CA, USA | F | Adhesion molecule expressed on endothelial cells | Small and large vessels Endothelial progenitor cells |
| Polyclonal goat anti-rat/mouse vascular endothelial growth factor (VEGF) (P-20; sc-1836) | 1:100, Santa Cruz | Biotinylated anti-goat IgG; Zymed Laboratories, San Francisco, CA, USA | F and MC tested | VEGF | VEGF |
| Polyclonal rabbit anti-rat/mouse vascular endothelial growth factor (VEGF) (147; sc-507) | 1:100, Santa Cruz | Biotinylated rabbit anti-mouse IgG; Zymed Laboratories, San Francisco, CA, USA | F and MC tested | VEGF | VEGF |
| Polyclonal rabbit anti-mouse/rat hypoxia-inducible factor (HIF)-1α (H-206, sc10790) | 1:100, Santa Cruz | Biotinylated rabbit anti-mouse IgG; Zymed Laboratories, San Francisco, CA, USA | MC | HIF-1α | HIF-1α |