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. 2020 Apr 4;56:1–18. doi: 10.1540/jsmr.56.1

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©2020 The Japan Society of Smooth Muscle Research

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2. — Visualisation of NG2 chondroitin sulphate proteoglycan (NG2)-positive mural cells in precapillary arterioles (PCAs).

Double immunostaining for NG2 (green) and α-smooth muscle actin (α-SMA, red) combined with nuclear staining (blue) reveals the round cell bodies of mural cells (arrows) in the PCAs of rat rectal submucosa (A–C). Smooth muscle cells in the connecting larger arteriole (asterisks) are also faintly immunopositive for NG2. A bladder mucosal specimen of NG2 DsRed mouse demonstrates the NG2-expressing suburotherial microvasculature consisting of a branching arteriolar tree (a), PCAs (pca) and a capillary meshwork (cap) (D). Mural cells in the PCA shows an oval or round cell body and circumferentially-oriented processes (E). Micrographs in A–C are reproduced from (17), and those in D and E are from (19) with permission.