Fig. 4.

G-patch binding stabilizes a DHX15 conformation consistent with RNA binding. (A and C) Different conformations of ctPrp43 recorded in the presence (A) or absence (C) of RNA are shown [PDB ID codes 5lta and 5ltk (17)]. The location of G-patch sites 1 and 2 on Prp43 according to superpositions with the DHX15-G-patch complex are indicated by dashed circles. RNA and ADP present in A are shown in black. The location of the RNA binding channel as displayed in A is indicated in C by dashed lines. Rotation of the RecA domains necessary to transform the RNA-bound structure into the RNA-free structure is indicated by an arrow. (B and D) Superpositions of the DHX15–G-patch complex with the structures shown in A and C, respectively.