Fig. 2.

GUSi reduces the acute toxicity exerted by irinotecan treatment. Numbers of BrdU+ cells in 10 consecutive crypts were blindly quantified in the (A) ileum, (B) proximal colon, and (C) distal colon. Error bars are ± SEM. *P < 0.05 by one-way ANOVA with Sidak’s multiple comparisons test; **P < 0.01 by one-way ANOVA with Sidak’s multiple comparisons test. Immunohistochemistry to detect BrdU+ cells (brown) in (D) proximal (quantified in B) and (E) distal (quantified in C) halves of colons of mice treated as indicated; nuclei are counterstained with hematoxylin (blue). (Scale bar, 50 μm.)