Fig. 2.

Elesclomol regulates the contractility of myofibroblasts. (A) Representative double immunofluorescence images of apoptotic myofibroblasts incubated with F-actin (green) and α-SMA antibody (red). The results show that the TGF-β1-induced upregulation of α-SMA was inhibited by 10 μM elesclomol (n = 9). Scale bar, 100 μm. (B) Quantification of α-SMA-positive cells. (C) Effect of 10 μM elesclomol on the contractility of TGF-β1-induced fibroblasts. (D) The ratio of the gel outline to the well outline was taken as a measure of contraction strength. The results are expressed as mean ± SD (n = 3). **P < 0.01 vs. control, ##P < 0.01 vs. TGF-β1.