Fig. 4.

Elesclomol inhibits HS formation in rabbit ears. (A) Schematic diagram of rabbit ear HS model. (B) Chemical structure of elesclomol (C₁₉H₂₀N₄O₂S₂; molecular weight, 400.52). (C) Photographs of the full-thickness skin wounds (wound diameter on day 0, 8 mm). (D) HE staining of scar tissue at 35 days after wounding. The dotted circles indicate the cross-sectional area of the HS. Scale bar, 200 μm. (E) Masson staining of scar tissue at 35 days after wounding. “H” indicates the scar thickness, while “h” indicates the normal skin thickness. Scale bar, 200 μm. Higher magnification images within the yellow dotted box show the difference of collagen fibers. Scale bar, 50 μm. (F) The scar elevation index was significantly lower in the elesclomol group than in the vehicle and untreated groups. Data are expressed as mean ± SD (n = 6). **P < 0.01 vs. control.