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. 2020 Mar 20;23(4):100997. doi: 10.1016/j.isci.2020.100997

Figure 4.

Figure 4

Rab25 Binds and Prevents EGFR Degradation

(A) Images of confocal microscopy of A549R cells stained with antibodies of Rab25 and EGFR and DAPI. White arrows indicate the merged signals. Scale bar of right picture is 2.5 μm.

(B) Rab25 was immunoprecipitated from CNE2R cells, followed by immunoblotting of EGFR and Rab25.

(C) CNE2R cells treated with sham or 5 Gy radiation for 24 h were stained with EGFR and Rab25 antibodies and subjected to Duolink in situ PLA. Scale bar, 25 μm. Bar diagrams, the number of interactions per cell. Mean ± SD, ∗∗p < 0.01.

(D) Images of confocal microscopy of cells stained with antibodies of EEA1 and EGFR.

(E) Co-localization of EGFR and early endosome marker EEA1. Mean ± SD, ∗∗p<0.01.

(F) Immunoblotting of EGFR in CNE2R cells transfected with control or shRab25 and treated with either vehicle (0.01% dimethyl sulfoxide) or 10uM cycloheximide (Chx) for the indicated time.