Figure EV1. DICER‐KO B cells form mature IS with OT‐II‐isolated CD4⁺ T cells in the presence of OVA.

• A
  Model of IS in vitro formation between DICER‐KO B cells and OTII‐derived CD4⁺ T cells.
• B, C
  Representative flow cytometry analysis of CD4⁺ T‐cell proliferation assessed by Ki67 expression (B) and CFSE labeling of T cells (C) in the presence or absence of OVA after 72 h co‐culture with DICER‐KO B cells. Bar charts represent the Mean values ± SEM of at least four independent experiments. Significance was assessed by paired Student's t test comparing the OVA and NO OVA conditions; *P < 0.05.