Experimental timelines for apyrase administration and behavioral study.
The effects of apyrase (40, 80, and 160 U/ml) infusion into cerebral ventricle on social interaction time (n = 8, 10, 10, 10 mice; Interaction F
3,34 = 3.917, P = 0.0167; Target F
1,34 = 0.0004, P = 0.9834; Drug F
3,34 = 2.295, P = 0.0954; for target, Apyrase 80 U/ml versus control, P = 0.0008, two‐way ANOVA with Tukey's post‐test).
The effects of apyrase (40, 80, and 160 U/ml) infusion into cerebral ventricle on sucrose preference (n = 8, 10, 9, 11 mice; Treatment F
3,34 = 2.87, P = 0.0505; Apyrase 80 U/ml versus control, P = 0.007, one‐way ANOVA with Fisher's LSD test).
Experimental timelines for apyrase/ATP administration and behavioral tests.
The effect of apyrase (40 U/ml) infusion into hippocampal on the social interaction time (n = 14, 20 mice; Interaction F
1,32 = 7.0754, P = 0.0121; Target F
1,32 = 0.68447, P = 0.4142; Drug F
1,32 = 18.211, P = 0.0002; Apyrase versus control, P < 0.0001, Student's t‐test).
The effect of apyrase (40 U/ml) infusion into hippocampal on the sucrose preference (n = 10, 16 mice; Apyrase versus control, P = 0.0003, Student's t‐test).
The social avoidance behavior in mice with ATP intra‐hippocampal infusion after apyrase exposure (n = 14, 10, 10 mice; Interaction F
2,31 = 2.051, P = 0.1457; Target F
1,31 = 2.666, P = 0.1127; Drug F
2,31 = 3.711, P = 0.0359; for Target, Apyrase‐ACSF versus Vehicle, P = 0.0387; Apyrase‐ATP versus Apyrase‐ACSF, P = 0.0099, two‐way ANOVA with Tukey's post‐test).
The sucrose preference in mice with ATP intra‐hippocampal infusion after apyrase exposure (n = 10, 8, 8 mice; Treatment F
2,23 = 3.32, P = 0.0540; Apyrase‐ACSF versus Vehicle, P = 0.0466, one‐way ANOVA with Fisher's LSD test).
Data information: Data are expressed as the means ± SEM. NS
< 0.001.
