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. 2020 Mar 5;21(4):e47857. doi: 10.15252/embr.201947857

Figure 3. CD39 inhibition induces antidepressant‐like effects in adult mice.

Figure 3

  • A
    Experimental timelines for CSDS, ARL67156 administration, and behavioral study.
  • B
    The ATPase activity of mice exposure to ARL67156 (n = 7, 7, 5, 5 mice; Treatment F 3,20 = 16.81, P < 0.0001; CSDS – ACSF versus Ctrl – ACSF, P < 0.0001; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0405, one‐way ANOVA with Fisher's LSD test).
  • C
    The social avoidance behavior of susceptible mice with intracerebroventricular infusion of ARL67156 (n = 12, 10, 9, 9 mice; Interaction F 3,36 = 5.119, P = 0.0047; Target F 1,36 = 0.005, P = 0.9437; Drug F 3,36 = 3.626, P = 0.022; for Target, CSDS – ACSF versus Ctrl ‐ACSF, P = 0.0001; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0054, two‐way ANOVA with Tukey's post‐test).
  • D
    The sucrose preference of susceptible mice with intracerebroventricular infusion of ARL67156 (n = 12, 10, 10, 10 mice; Interaction F 1,38 = 5.352, P = 0.0262; Group F 1,38 = 5.711, P = 0.0219; Drug F 1,38 = 9.703, P = 0.0035; CSDS – ACSF versus Ctrl – ACSF, P = 0.0083; Ctrl – ARL67156 versus CSDS – ACSF, P = 0.0027; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0031, two‐way ANOVA with Tukey's post‐test).
  • E, F
    The social avoidance behavior (E) and sucrose preference (F) in mice with intra‐hippocampal infusion of ARL67156 after CSDS (n = 9, 9, 9, 8 mice; for social behavior, Interaction F 3,31 = 5.579, P = 0.0035; Target F 1,31 = 0.5121, P = 0.4796; Drug F 3,31 = 8.367, P = 0.0003; CSDS – ACSF versus Ctrl – ACSF, P < 0.0001; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0131; sucrose, Interaction F 1,30 = 0.3342, P = 0.5675; Group F 1,30 = 17.22, P = 0.0003; Drug F 1,30 = 0.05903, P = 0.8097; CSDS‐ ACSF versus Ctrl – ACSF, P = 0.0761, two‐way ANOVA with Tukey's post‐test).
  • G
    The immobility time in the TST of mice with intra‐hippocampal infusion of ARL67156 after CSDS (n = 16, 12, 21, 19 mice; Treatment F 3,64 = 13.61, P < 0.0001; CSDS – ACSF versus Ctrl – ACSF, P = 0.0059; Ctrl – ARL67156 versus Ctrl – ACSF, P = 0.0011; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0002, one‐way ANOVA with Fisher's LSD test).
  • H
    The immobility time in the FST with intra‐hippocampal infusion of ARL67156 after CSDS (n = 21, 12, 23, 19 mice; Treatment F 3,71 = 12.94, P < 0.0001; CSDS – ACSF versus Ctrl – ACSF, P = 0.0036; Ctrl – ARL67156 versus Ctrl – ACSF, P = 0.0428; CSDS – ARL67156 versus CSDS – ACSF, P < 0.0001, one‐way ANOVA with Fisher's LSD test).
Data information: Data are expressed as the means ± SEM. NS P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. Ctrl, control.Source data are available online for this figure.