Figure EV3. The effects of ARL67156 on locomotor activity and anxiety behaviors of mice.

• A
  The locomotor activity of susceptible mice with ARL67156 (100 μM) infusion into the right cerebral ventricle in the open field test (n = 8, 6, 6, 7 mice; Interaction F _1,23 = 0.3174, P = 0.5786; Group F _1,23 = 6.619, P = 0.0170; Drug F _1,23 = 4.226, P = 0.0513, two‐way ANOVA with Tukey's post‐test).
• B
  The locomotor activity of mice with ARL67156 (100 μM) infusion into the hippocampus in the open field test (n = 9, 9, 9, 8 mice; Interaction F _1,31 = 0.1670, P = 0.6856; Group F _1,31 = 0.8477, P = 0.3643; Drug F _1,31 = 0.01314, P = 0.9095, two‐way ANOVA with Tukey's post‐test).
• C
  The distance spent in center zone of stressed mice with ARL67156 (100 μM) in the OFT (n = 13, 9, 16, 18 mice; Treatment F _3,52 = 1.880, P = 0.1444; CSDS – ACSF versus Ctrl – ACSF, P = 0.0346; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0671, one‐way ANOVA with Fisher's LSD test).
• D, E
  The anxiety behaviors of stressed mice with ARL67156 (100 μM) accessing by the open arms (D) and the closed arms (E) in the EPM test (n = 13, 8, 17, 16 mice; for the open arms, Treatment F _3,50 = 1.569, P = 0.2086; CSDS – ACSF versus Ctrl – ACSF, P = 0.0891; CSDS – ARL67156 versus CSDS – ACSF, P = 0.1234; for the closed arms, Treatment F _3,50 = 1.063, P = 0.3731, one‐way ANOVA with Fisher's LSD test).

Data information: Data are expressed as the means ± SEM. NS P > 0.05; *P < 0.05. Ctrl, control.Source data are available online for this figure.