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. 2020 Mar 5;21(4):e47857. doi: 10.15252/embr.201947857

Figure EV5. The effect of extracellular recombinant CD39 protein (CD39Fc) on depressive behaviors of stressed mice infusion with LV‐siCD39.

Figure EV5

  • A
    Experimental timelines.
  • B
    Expression of CD39 in the hippocampus of mice injected with CD200Fc (n = 6 mice/group; CD39Fc versus IgG, P = 0.0004, Student's t‐test).
  • C
    Time in the interaction zone of mice infusion with CD39Fc after knockdown of CD39 in SI test (n = 13, 14, 16, 11 mice; Interaction, F 3,100 = 2.977, P = 0.0352; Target, F 1,100 = 6.175, P = 0.0146; Drug, F 3,100 = 2.028, P = 0.1148; for target, CSDS‐LV‐GFP‐IgG versus Ctrl‐LV‐GFP‐IgG, P = 0.0077; CSDS‐LV‐siCD39‐IgG versus CSDS‐LV‐GFP‐IgG, P = 0.0031; CSDS‐LV‐siCD39‐CD39Fc versus CSDS‐LV‐siCD39‐IgG, P = 0.0378, two‐way ANOVA with Tukey's post‐test).
  • D
    Social interaction ratio of mice infusion with CD39Fc after knockdown of CD39 in SI test (n = 13, 14, 16, 11 mice; Treatment, F 3,50 = 5.690, P = 0.0020; CSDS‐LV‐GFP‐IgG versus Ctrl‐LV‐GFP‐IgG, P = 0.0012; CSDS‐LV‐siCD39‐IgG versus CSDS‐LV‐GFP‐IgG, P = 0075; CSDS‐LV‐siCD39‐CD39Fc versus CSDS‐LV‐siCD39‐IgG, P = 0.0263, one‐way ANOVA with Fisher's LSD test).
  • E, F
    Infusion with CD39Fc into hippocampus abolished the decreased immobility time of stressed mice injection with LV‐siCD39 in the TST (E) and FST (F) (for TST, n = 13, 14, 13, 11; Treatment, F 3,47 = 13.22, P < 0.0001; CSDS‐LV‐GFP‐IgG versus Ctrl‐LV‐GFP‐IgG, P < 0.0001; CSDS‐LV‐siCD39‐IgG versus CSDS‐LV‐GFP‐IgG, P < 0001; CSDS‐LV‐siCD39‐CD39Fc versus CSDS‐LV‐siCD39‐IgG, P = 0.05; for FST, n = 13, 14, 16, 11; Treatment, F 3,50 = 4.047, P = 0.0119; CSDS‐LV‐GFP‐IgG versus Ctrl‐LV‐GFP‐IgG, P = 0.0116; CSDS‐LV‐siCD39‐IgG versus CSDS‐LV‐GFP‐IgG, P = 0191; CSDS‐LV‐siCD39‐CD39Fc versus CSDS‐LV‐siCD39‐IgG, P = 0.0261, one‐way ANOVA with Fisher's LSD test).
  • G
    Total distance of mice in the OFT (n = 14, 14, 15, 11 mice; Treatment, F 3,50 = 1.136, P = 0.3437, one‐way ANOVA with Fisher's LSD test).
Data information: Data are expressed as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure.