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. 2020 Mar 5;21(4):e47857. doi: 10.15252/embr.201947857

Figure 6. Inhibition and knockdown of CD39 in the hippocampus rescue stress‐induced stubby spine loss.

Figure 6

  • A
    Representative confocal z‐stack images of LV‐infected dendritic segments in the DG granule neurons from control and susceptible mice with different treatment. White arrows indicate stubby spines, yellow arrows indicate mushroom spines, and blue arrows indicate thin spines.
  • B–E
    Quantification of total (B), stubby (C), mushroom (D), and thin (E) spine density in DG from control and susceptible mice infected with LV‐GFP or LV‐siCD39 (n = 15 segments from 4 mice/group; for total density: Treatment F 2,42 = 3.12, P = 0.0544; Ctrl – LV‐GFP versus CSDS – LV‐siCD39, P = 0.0312; CSDS – LV‐siCD39 versus CSDS – LV‐GFP, P = 0.0425; stubby density: Treatment F 2,42 = 10.93, P = 0.0002; P = 0.0026; CSDS – LV‐CD39 versus CSDS – LV‐GFP, P < 0.0001; mushroom density: Treatment F 2,42 = 0.34, P = 0.7161; thin density: F 2,42 = 1.00, P = 0.3758, one‐way ANOVA with Fisher's LSD test).
  • F
    Representative confocal z‐stack images of LV‐infected dendritic segments in the DG granule neurons from control and susceptible mice with different treatment. White arrows indicate stubby spines, yellow arrows indicate mushroom spines, and blue arrows indicate thin spines.
  • G–J
    Quantification of total (G), stubby (H), mushroom (I), and thin (J) spine density in DG from control and susceptible mice treatment with ACSF or ARL67156 (n = 11, 13, 10 segments from 2, 4, 2 mice, respectively; for total density: Treatment F 2,31 = 4.474, P = 0.0196; CSDS – ASCF versus Ctrl – ACSF, P = 0.0366; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0086; stubby density: Treatment F 2,31 = 3.327, P = 0.0491; CSDS – ASCF versus Ctrl – ACSF, P = 0.1026; CSDS – ARL67156 versus CSDS – ACSF, P = 0.0179; mushroom density: Treatment F 2,31 = 1.986, P = 0.1543; thin density: F 2,31 = 1.475, P = 0.2445, one‐way ANOVA with Fisher's LSD test).
Data information: Data are expressed as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure.