Figure 2. HOXA4 negatively regulates YAP/TEAD transcriptional activity independently of YAP phosphorylation.

• A
  Quantitative real‐time PCR analysis of CTGF, CYR61, and BIRC5 in HEK 293T cells with knockdown of endogenous HOXA4. ShLATS1/2 and shYAP were used as an experimental control. *P < 0.05, **P < 0.01 versus shCtrl, by ANOVA with Sidak's correction.
• B
  8×GTIIC‐luciferase reporter assays in HEK 293T cells transfected HOXA4 and/or shLATS1/2. **P < 0.01 versus shCtrl, by two‐way ANOVA with Sidak's correction.
• C
  Quantitative real‐time PCR analysis of HOXA4, YAP, CTGF, and CYR61 in HEK 293T cells with overexpression of HOXA4 and/or constitutive active form of YAP (YAP ‐5SA). *P < 0.05, **P < 0.01 versus YAP‐5SA with LacZ, by ANOVA with Sidak's correction.
• D
  Quantitative real‐time PCR analysis of HOXA4, YAP, CTGF, and CYR61 in HEK 293T cells with knockdown of endogenous HOXA4 and/or overexpression of YAP‐5SA. *P < 0.05, **P < 0.01 versus YAP‐5SA with shCtrl, by ANOVA with Sidak's correction.

Data information: All data are presented as mean ± SEM; three biological repeats.