Figure 3. Protein–protein interactions between HoxA4 and TEADs.

• A, B
  Association of HOXA4 with TEAD1 (A) but not YAP (B) indicated by co‐IP experiments. HEK 293T cells were co‐transfected with FLAG‐HOXA4 and Myc‐TEAD1 or Myc‐YAP.
• C
  Interaction between endogenous TEAD1 and HOXA4 by co‐IP experiments in HEK 293T cells. The interaction between endogenous TEAD1 and YAP was used as a positive control.
• D
  Immunoprecipitation using FLAG antibody in lysates from HEK 293T cells transfected with plasmids expressing HOXA4 truncation mutants and TEAD1.
• E
  Immunoprecipitation using FLAG antibody in lysates from HEK 293T cells transfected with plasmids expressing TEAD1 truncation mutants and HOXA4. Arrowheads indicate IgG heavy chain. Arrows indicate TEAD1 (a.a. 1–426).
• F
  Requirement of TEAD1 (a.a. 30–135) for its interaction with HOXA4 via its homeodomain (a.a. 216–274).
• G
  Failure of TEAD1 (a.a. 30–102), TEA domain alone, to interact with HOXA4.
• H, I
  Diagram showing the structure of truncated HOXA4 (H) and TEAD1 (I) constructs and possible protein–protein interactions between them. Conservation between human TEAD1, TEAD2, TEAD3, and TEAD4 is shown (I).

Data information: All experiments above were repeated at least twice.