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. 2020 Mar 9;21(4):e48389. doi: 10.15252/embr.201948389

Figure 4. HOXA4 negatively regulates TEAD‐YAP interaction.

Figure 4

  • A
    ChIP assays of CTGF and CYR61 promoters using TEAD4 or YAP antibodies in HEK 293T cells transfected with plasmids expressing HOXA4. *P < 0.05, **P < 0.01, by unpaired two‐tailed Student's t‐test. Data are expressed as the means ± SEM of three independent experiments.
  • B
    Competitive co‐IP assay and densitometry showing significantly reduced TEAD‐YAP interaction by overexpression of HOXA4 in a dose‐dependent manner in HEK 293T cells. *P < 0.05, **P < 0.01, by ANOVA with Sidak's correction. Data are presented as mean ± SEM of three independent experiments.
  • C
    Working hypothesis whereby HOXA4 inhibits YAP/TEAD‐mediated transcription activity.