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. 2020 Mar 9;21(4):e48389. doi: 10.15252/embr.201948389

Figure EV4. Cellular function of HOXA4 in vascular smooth muscle cells.

Figure EV4

  • A
    FANTOM5 CAGE data showing higher expression levels of HOXA4 in human VSMCs.
  • B
    Representative immunofluorescent staining images for α‐SMA and HOXA4 on human aortic samples. Scale bars indicate 20 μm.
  • C
    Expression of Hoxa4 and markers of VSMC proliferation and contractile phenotype in mouse primary aortic smooth muscle cells stimulated with PDGF‐BB. All data are presented as mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, by unpaired two‐tailed Student's t‐test.
  • D
    Western blotting analysis of cytosolic and nuclear protein levels of HOXA4, YAP, TAZ, and TEAD1 in human VSMCs.
  • E
    Immunocytochemistry of human VSMCs with anti‐HOXA4 antibody. Scale bars indicate 50 μm.
  • F, G
    Representative images of BrdU incorporation assay in human VSMCs transduced with the indicated genes (related to Fig 6H and J). Scale bars indicate 200 μm.