-
A
Representative Western blotting analysis of total protein lysates of primary human VSMCs transduced with lentiviral vectors encoding shRNAs of HOXA4 and TEAD1/3/4. Quantification of alpha‐smooth muscle actin and calponin protein levels is expressed as the means ± SEM of three independent experiments. *P < 0.05, **P < 0.01, by ANOVA with Sidak's correction.
-
B
Proliferation assay in VSMCs transduced with indicated genes. Virus‐transfected human VSMCs were plated at equal density in growth medium, and then, cell viability was analyzed by Cell Counting Kit‐8 at indicated days at each time point as indicated. *P < 0.05 versus shCtrl, by ANOVA with Sidak's correction. Data are presented as mean ± SEM of three independent experiments.
-
C
Representative immunoblotting images and quantification of the interaction between endogenous TEAD1 and HOXA4 or YAP using co‐IP experiments in human VSMCs with knockdown of HOXA4. *P < 0.05 versus shCtrl, by ANOVA with Sidak's correction. Data are presented as mean ± SEM of three independent experiments.
-
D
ChIP assays of CTGF, CYR61, and α‐SMA promoters using anti‐FLAG‐tag, anti‐Myc‐tag, and anti‐TEAD1 antibodies in human VSMCs transfected with plasmids expressing Myc‐tagged YAP‐5SA and/or FLAG‐tagged HOXA4. *P < 0.05, **P < 0.01 versus FLAG‐GFP, by unpaired two‐tailed Student's t‐test. Data are expressed as the means ± SEM of three independent experiments.
