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. 2020 Feb 7;21(4):e49700. doi: 10.15252/embr.201949700

Figure 1. Epithelial cells round up and downregulate adherens junctions at mitosis.

Figure 1

  • A
    Dynamic regulation of fluorescently tagged beta‐catenin/Armadillo (Arm‐GFP) during mitosis in the growing fly wing epithelium. Notice downregulation of Arm‐GFP as cells round up for mitosis (arrow). Scale bar ˜2 μm. n > 10 independent biological replicates.
  • B
    Dynamic regulation of fluorescently tagged E‐cadherin (E‐cad‐GFP) during mitosis in the growing fly wing epithelium. Notice downregulation of E‐cad‐GFP as cells round up for mitosis (arrow). Scale bar ˜2 μm. n > 10 independent biological replicates.
  • C
    E‐cad‐GFP remains downregulated in cells arrested in mitosis with colchicine. Scale bar ˜2 μm. n > 10 independent biological replicates.
  • D
    Quantification of the degree of downregulation of Arm‐GFP and E‐cad‐GFP at the junction between mitotic cells and their interphase neighbours (n > 10 independent samples per cell cycle stage). Mean ± 1 SD shown.
  • E
    Two adjacent mitotic cells show no detectable Arm‐GFP at the junction between them. Scale bar ˜2 μm.
  • F–I
    Electron microscopy cross‐sections of wing epithelial cells at interphase, prophase, prometaphase and telophase. Notice adherens junctions (dark staining, highlighted in insets) are downregulated as cells round up for mitosis. Scale bars ˜1 μm (low mag.) and ˜10 nm (high mag.). n > 10 independent biological replicates. Asterisks indicate a single mitotic cell. Green arrowheads indicate adherens junctions.