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. 2020 Feb 7;21(4):e49700. doi: 10.15252/embr.201949700

Figure EV1. Ectopic activation of Rho is sufficient to drive cell rounding and downregulate adherens junctions in the Drosophila wing disc.

Figure EV1

  1. Control third instar wing imaginal disc stained for F‐actin (phalloidin, red) and E‐cadherin (magenta). Note absence of clones (GFP), there is no background signal for GFP. Scale bar ˜20 μm. n > 4 independent biological replicates.
  2. Induction of clones expressing UAS.GFP and UAS.RhoV14 causes dramatic increases in F‐actin and co‐incident decreases in E‐cadherin, along with abnormal tissue morphology. High‐magnification view (bottom) shows reduced E‐cadherin and rounded shape of cells expressing RhoV14. Scale bars ˜20 μm (low mag) ˜10 μm (high mag). n > 4 independent biological replicates.