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. 2020 Feb 7;21(4):e49700. doi: 10.15252/embr.201949700

Figure 3. Loss of RhoGEF ECT2/Pbl prevents mitotic rounding and causes cell extrusion and apoptosis.

Figure 3

  1. Nuclear localised Pbl‐GFP becomes cytoplasmic in mitotic cells, co‐incident with Rok‐GFP re‐localisation to the plasma membrane. Scale bar ˜1 μm. n > 5 independent biological replicates.
  2. Phosphorylated Myosin‐II regulatory light chain (p‐MLC; purple) detected by antibody staining reveals high levels around the cortex of rounded mitotic cells in wild‐type wing epithelial cells, but not those expressing UAS.pbl‐RNAi. Phospho‐Histone H3 (pH3) staining (green) marks mitotic cell chromosomes. Quantification shown right (n > 10 independent samples per genotype; mean ± 1 SD shown). Scale bar ˜1 μm.
  3. A stripe of expression of ptc.Gal4 UAS.pbl‐RNAi in the wing disc leads to both enlarged cells and many basally extruded cells that undergo apoptosis, marked by pyknotic nuclei and loss of aPKC/Dlg staining. Scale bars ˜20 μm. n > 6 independent biological replicates.