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. 2020 Feb 7;21(4):e49700. doi: 10.15252/embr.201949700

Figure EV4. RNAi of both ECT2/Pbl and beta‐Pix leads to reduced apical ZO‐1 immunostaining in human Caco2 cells.

Figure EV4

  1. siRNA knockdown of ECT2 and beta‐PIX individually does not affect the localisation of ZO‐1, while double siRNA causes discontinuous ZO‐1 localisation in most cells. Scale bar ˜10 μm. n > 3 independent biological replicates.
  2. Quantification of (A). n > 3 independent biological replicates. Mean ± SEM shown.
  3. Transepithelial resistance assay for measuring tight junction function in each of the siRNA conditions in (A). n > 3 independent biological replicates. Mean ± SEM shown.