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. 2020 Feb 7;21(4):e49700. doi: 10.15252/embr.201949700

Figure 5. Mutation of Aurora A traps rounded cells in a delayed and extended mitosis, while chemical inhibition of both Aurora A and B prevents mitotic rounding.

Figure 5

  1. Phosphorylated Myosin‐II regulatory light chain (p‐MLC; purple) detected by antibody staining reveals high levels around the cortex of rounded mitotic cells (pH3, red) in wild‐type wing discs, and aurA homozygous mutant wing discs, but not upon inhibition of both AurA and AurB by treatment with VX‐680. Note increased numbers of mitotic cells in aurA homozygous mutant wing discs, due to delayed mitotic progression. Scale bars ˜20 μm (low mag.) and ˜1 μm (high mag.). n > 15 independent biological replicates.
  2. Live imaging of MyoII‐GFP reveals delayed mitosis in aurA homozygous mutant wing discs, while inhibition of both AurA and AurB by treatment with VX‐680 leads to complete loss of mitotic rounding and MyoII‐GFP membrane localisation. Asterisks indicate a single mitotic cell. Scale bars ˜1 μm. Quantification shown on the bottom right (n = 5 independent samples per genotype).
  3. Live imaging of E‐cad‐GFP reveals delayed mitosis in aurA homozygous mutant wing discs, cytokinesis failure in UAS.aurB‐RNAi expressing wing discs, and complete failure of mitotic rounding and E‐cad‐GFP downregulation upon inhibition of both AurA and AurB by treatment with VX‐680. Asterisks indicate a single mitotic cell. Scale bars ˜1 μm. Quantification shown on the bottom right (n = 9 independent samples per genotype).