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. 2020 Feb 7;21(4):e49700. doi: 10.15252/embr.201949700

Figure 6. Genetic redundancy between Aurora A and B kinases in vivo .

Figure 6

  1. Striped ptc.Gal4‐driven expression of UAS.GFP in a control wing imaginal disc. Scale bars ˜20 μm. n > 6 independent biological replicates.
  2. Striped ptc.Gal4‐driven expression of UAS.GFP in an aurA homozygous mutant wing imaginal disc leads to increased numbers of pH3‐positive mitotic cells. n > 7 independent biological replicates.
  3. Striped ptc.Gal4‐driven expression of UAS.GFP and UAS.aurB‐RNAi in a control wing imaginal disc leads to enlarged cells with large nuclei (marked by DAPI; white arrowhead). n > 6 independent biological replicates.
  4. Striped ptc.Gal4‐driven expression of UAS.GFP and UAS.aurB‐RNAi in an aurA homozygous mutant wing imaginal disc leads to extrusion and apoptosis of cells (marked by Dcp1 and pyknotic nuclei). n > 8 independent biological replicates.
  5. Striped ptc.Gal4‐driven expression of UAS.GFP and UAS.pbl‐RNAi in the wing imaginal disc leads to extrusion and apoptosis of cells (marked by Dcp1 and pyknotic nuclei). n > 8 independent biological replicates.
  6. ptc.Gal4‐driven expression of UAS.aurB‐RNAi in an aurA homozygous mutant endoreplicating salivary gland has no phenotypic consequence. n > 6 independent biological replicates.
  7. MARCM clones (GFP‐positive) expressing UAS.aurB‐RNAi in an aurA homozygous mutant cells result in single‐cell clones (i.e. no cell division), while individual loss of AurA or AurB allows some cell proliferation. Quantification shown on the right (n > 7 independent samples per genotype; mean ± 1 SD shown). Scale bar ˜20 μm.