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. 2020 Feb 7;21(4):e49700. doi: 10.15252/embr.201949700

Figure EV6. Overexpression of ECT2/Pbl in the wing imaginal disc leads to ectopic spreading of aPKC and cell rounding in the Drosophila wing disc.

Figure EV6

  1. Control third instar wing disc stained for aPKC and nuclei (DAPI). Scale bar ˜20 μm. n > 3 independent biological replicates.
  2. MS1096.Gal4‐driven UAS.Cdc42 V12 causes ectopic spreading of aPKC and abnormal tissue morphology. Scale bar ˜20 μm. n > 6 independent biological replicates.
  3. MS1096.Gal4‐driven UAS.Pbl causes ectopic spreading of aPKC and abnormal tissue morphology. Scale bar ˜20 μm. n > 8 independent biological replicates.
  4. High‐magnification cross‐section of MS1096.Gal4‐driven UAS.Pbl causing ectopic spreading of aPKC and abnormal tissue morphology. The phenotypes of Cdc42 and Pbl gain of function are similar in the wing disc. Scale bar ˜2 μm. n > 4 independent biological replicates.