Figure EV2. YTHDF1 is dispensable for intestinal development.

1. Generation of Ythdf1 conditional knockout (Ythdf1 ^cKO) mouse.
2. Genotyping of wild‐type (Ythdf1 ^CTL), heterogeneous (Ythdf1 ^Heter), and Ythdf1 ^cKO mice.
3. Immunoblot analysis of intestinal epithelia from Ythdf1 ^CTL and Ythdf1 ^cKO mice.
4. Bodyweight of Ythdf1 ^CTL and Ythdf1 ^cKO mice at the age of 2 months. Data are represented as mean ± SEM. (15 mice for each group, t‐test)
5. H&E staining of duodenum, ileum, and colon from wild‐type (Ythdf1 ^CTL) and Ythdf1 ^cKO mice.
6. The quantification of crypt height in (E). Scale bar, 50 μm. Data are represented as mean ± SEM. (9 mice for each group, t‐test)
7. BrdU staining of duodenum, ileum, and colon from wild‐type (Ythdf1 ^CTL) and Ythdf1 ^cKO mice.
8. The quantification of BrdU⁺ cells per crypt in (G). Scale bar, 50 μm. Data are represented as mean ± SEM. (9 mice for each group, t‐test)
9. Alkaline phosphatase staining (ALPI) and Alcian blue staining (Alcian blue) of small intestine from wild‐type (Ythdf1 ^CTL) and Ythdf1 ^cKO mice. Scale bar, 50 μm.

Source data are available online for this figure.