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. 2020 Feb 17;21(4):e49229. doi: 10.15252/embr.201949229

Figure EV3. The role of m6A in the maintenance of intestinal stemness.

Figure EV3

  1. Morphology of organoids from wild‐type (Ythdf1 CTL) and Ythdf1 cKO mice at day 6. Scale bar, 250 μm.
  2. Quantification of percentage of organoid budding in (A) (3 independent replicates).
  3. Immunoblot analysis of organoids infected with lentivirus expression Flag‐tagged YTHDF1 (Flag‐Y1) and YTH domain‐deleted mutant (Flag‐Y1‐mut).
  4. Immunoblot analysis of organoids with or without METTL3 knockdown.
  5. Morphology of organoids in Wnt3a‐conditioned medium with or without METTL3 knockdown. Scale bar, 200 μm.
  6. Quantification of differentiated versus undifferentiated organoids from (E). Data are represented as mean ± SEM. Three biological replicates.
  7. Spheroid diameter analysis from (E). Data are represented as mean ± SEM. ***P < 0.001 (3 biological replicates, t‐test).

Source data are available online for this figure.