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. 2020 Feb 17;21(4):e49229. doi: 10.15252/embr.201949229

Figure 4. YTHDF1 is required for maintenance of intestinal stem cells.

Figure 4

  1. Fluorescent in situ hybridization for Ythdf1 mRNA in Ythdf1 CTL and Ythdf1 cKO small intestines. Scale bar, 50 μm.
  2. Immunoblot analysis of fractionated murine intestine enriched for villus cells and crypt cells.
  3. Flow cytometry analysis of YTHDF1 protein expression in GFPhigh/low/neg populations from Lgr5‐GFP‐IRES‐creERT2 mice.
  4. Morphology of organoids from Lgr5‐creERT2:Ythdf1 fl/fl mice in Wnt3a‐conditioned medium without or with 4‐OHT induction and infected with lentivirus expressing YTHDF1 or YTHDF1 mutant. Scale bar, 250 μm.
  5. Quantification of differentiated versus undifferentiated organoids from (D). Data are represented as mean ± SEM. **P < 0.01 (4 biological replicates, t‐test).
  6. Relative mRNA levels of intestinal stem cell and differentiation marker genes in organoids described in (D). Data are represented as mean ± SEM. 4 biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001 (t‐test).

Source data are available online for this figure.