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. 2020 Feb 17;21(4):e49229. doi: 10.15252/embr.201949229

Figure 6. TCF7L2 is a key functional target of YTHDF1.

Figure 6

  1. Immunoblot analysis of HCT116 cells with YTHDF1 knockdown. Two major bands were detected for human TCF7L2 due to alternative splicing.
  2. Polysome profiles of HCT116 cells with or without YTHDF1 knockdown. The right panels show the distributions of TCF7L2 and ACTB in polysome fractions. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01 (3 biological replicates, t‐test).
  3. Immunoblot analysis of crypts from Ythdf1 CTL and Ythdf1 cKO mice. Multiple bands were detected for mouse TCF7L2 representing different isoforms.
  4. Immunoblot analysis of mouse intestinal crypts treated with Wnt3a for 60 min.
  5. MeRIP‐qPCR analysis of m6A levels of TCF7L2 in crypts treated with or without Wnt3a. Data are represented as mean ± SEM. *P < 0.05 (3 biological replicates, t‐test).
  6. RIP analysis of the interaction of YTHDF1 with TCF7L2 mRNA. The enrichment was measured by qPCR and normalized to input. Data are represented as mean ± SEM. *P < 0.05 (3 biological replicates, t‐test).
  7. β‐catenin/TCF4 reporter activity in YTHDF1 knockdown cells infected with lentivirus expressing TCF7L2. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (3 biological replicates, t‐test).
  8. Morphology of organoids from Lgr5‐creERT2:Ythdf1 fl/fl mice in Wnt3a‐conditioned medium without or with 4‐OHT induction and infected with lentivirus expressing TCF7L2. Scale bar, 250 μm.
  9. Quantification of differentiated versus undifferentiated organoids from (H). Data are represented as mean ± SEM. **P < 0.01, ***P < 0.001 (3 biological replicates, t‐test).

Source data are available online for this figure.