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. 2020 Feb 11;21(4):e49113. doi: 10.15252/embr.201949113

Figure EV3. mtON increases the PMF and ATP synthesis light dose‐dependently.

Figure EV3

  1. Change in TMRE fluorescence with increasing light dose (fluence 0, 0.016, 0.08, 0.096, 0.16, 0.24 J/cm2). Two‐way ANOVA with Sidak's multiple comparisons test, +ATR: 0 versus 0.096 J/cm2 P = 0.0232, 0 versus 0.24 J/cm2 P = 0.0149, 0.016 versus 0.096 J/cm2 P = 0.0162, 0.016 versus 0.16 J/cm2 P = 0.0376, 0.016 versus 0.24 J/cm2 P = 0.0101. n = 3–6 mitochondrial preparations. Non‐linear dose–response fit carried out using GraphPad Prism. Data show mean ± standard deviation.
  2. ATP levels in response to increasing illumination normalized to maximum ATP synthesis given by succinate respiration (dotted line). Ten‐minute illumination time from Fig 2E. Two‐way ANOVA with Sidak's multiple comparison test, −ATR: succinate versus 1‐, 5‐, and 10‐min light each P < 0.0001. +ATR: succinate versus 1‐min light, P < 0.0001, 1‐min light versus 5‐min light *P = 0.0098, 1‐min light versus 10‐min light *P = 0.0001. n = 7 independent mitochondrial preparations. Data show mean ± SEM.