Figure 4.

ABI2 overexpression attenuates the promotive effect of miR-25 on PDAC cancer cells. (A) Aspc-1 cells were co-transfected with ABI2 vector and miR-25 mimics, and then RT-qPCR was carried out to detect the relative mRNA expression of ABI2. (B) CCK-8 assay was conducted to determine the proliferation of pretreated Aspc-1 cells. (C) EdU incorporation assay was performed and was monitored by microscopy (magnification, x100). Red indicates EdU-positive cell, Blue was stained for DAPI. The percentage of EdU-positive cells per optical field (at least 50 fields) was counted. (D) Determination of Aspc-1 cell cycle with flow cytometry. Values represent the mean ± SD. The experiments were repeated three times and conducted in triplicates. ^*P<0.05, ^**P<0.01 compared with control. miR, microRNA; ABI2, Abl interactor 2; EdU, 5-ethynyl-2'-deoxyuridine; NC, negative control.