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. 2020 Mar 6;19(5):3203–3210. doi: 10.3892/etm.2020.8574

Figure 2.

Figure 2

Downregulation of miR-106b-5p inhibited the proliferation and cell cycle progression of OS cells. (A) Saos-2 and U2OS cells transfected with the miR-NC or miR-106b-5p inhibitor were analyzed for miR-106b-5p expression via reverse transcription-quantitative PCR. (B) An MTT assay was performed to examine cell proliferation following transfection with the miR-NC or miR-106b-5p inhibitor. (C) Flow cytometry was performed to examine the cell cycle distribution of Saos-2 and U2OS cells following transfection with the miR-NC or miR-106b-5p inhibitor. Data are presented as the mean ± standard deviation. **P<0.01 and ***P<0.001 vs. NC group. miR, microRNA; OS, osteosarcoma; NC, negative control; OD, optical density.