Figure 4.

CDKN1A overexpression inhibits the proliferation and cell cycle progression of OS cells. (A) Reverse transcription-quantitative PCR and (B) western blotting were performed to examine the expression of CDKN1A in Saos-2 cells transfected with the blank pcDNA3.1 vector or pcDNA3.1-CDKN1A open reading frame plasmid. (C) An MTT assay was performed to examine cell proliferation and (D) flow cytometry was used to examine the cell cycle distribution. Data are presented as the mean ± standard deviation. ^***P<0.001 vs. pcDNA 3.1 group. CDKN1A, cyclin-dependent kinase inhibitor 1A; OS, osteosarcoma.