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. 2020 Mar 6;19(5):3203–3210. doi: 10.3892/etm.2020.8574

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Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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CDKN1A knockdown abolishes the suppressive effects of miR-106b-5p inhibitor transfection on OS cells. (A) Saos-2 cells were co-transfected with miR-106b-5p inhibitor and si-CDKN1A then reverse transcription-quantitative PCR and (B) western blot analysis was used to examine the expression levels of CDKN1A in Saos-2 cells. (C) MTT assay was used to analyze cell proliferation and (D) flow cytometry with propidium iodide staining was used to detect cell cycle distribution. ^**P<0.01 and ^***P<0.001 vs. miR-106b-5pinhibitor + si-NC group. CDKN1A, cyclin-dependent kinase inhibitor 1A; miR, microRNA; OS, osteosarcoma; si, small interfering; NC, negative control; OD, optical density.