Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 9;19(5):3275–3281. doi: 10.3892/etm.2020.8589

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Shang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Characterization of primary PASMCs isolated from pulmonary arteries of rats. (A) PASMCs isolated from the pulmonary artery mass and aggregated into clusters (5 days after start of culture). (B) PASMCs exhibiting long fusiform shapes and ‘peak-valley’ appearance (10 days after start of culture). (C) Immunocytochemistry staining confirmed α-smooth muscle actin expression based on the specific myofilament structure of PASMCs (15 days after start of culture). (D) Specific TMEM16A-siRNA lentiviral vectors, which also encode GFP, were transfected into PASMCs and observed under a light microscope. PASMCs, pulmonary artery smooth muscle cells; TMEM16A, transmembrane member 16A; siRNA, small interfering RNA.