Fig. 2.
RNA binding of U2AF2 RRM1,2-WT and RRM1,2-GS to weak and strong Py-tract RNA. (A) Residues in the RRM1,2 linker are replaced by Gly-Gly-Ser, and V254P to remove transient linker/RRM2 interactions. (B) Dissociation constants KD for the binding of RRM1,2-WT and RRM1,2-GS to strong (U9) and weak (U4A8U4) Py-tract RNAs determined by ITC. (C) NMR titrations showing spectral changes for residues in RRM2 that are located in proximity to the linker upon binding the weak Py-tract RNA. (D) Kinetic rates for the interaction of RRM1,2-WT and -GS with RNAs determined by switchSENSE.